Insertion junction: LMJ.RY0402.156716_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.156716
Chromosome:chromosome 6
Location:1566872
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g260400 FAP287 (1 of 1) PF13881 - Ubiquitin-2 like Rad60 SUMO-like (Rad60-SLD_2); Ubiquitin-like Flagellar Associated Protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCCATGTCTATCGGCAACTTCATCAGACG
Internal bar code:TCGGGCGCCGGCTTAAACCAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:517
LEAP-Seq percent confirming:82.4859
LEAP-Seq n confirming:146
LEAP-Seq n nonconfirming:31
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GTTCTACTGCGGAAACGCTC
Suggested primer 2:AGCTTAAAGGTGAGTGGCGA