| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.156736 |
| Chromosome: | chromosome 16 |
| Location: | 59728 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g695851 | (1 of 2) 2.1.1.229 - tRNA (carboxymethyluridine(34)-5-O)-methyltransferase / tRNA methyltransferase 9 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGAAGCCTGCATGTCCTGCATTTATCAC |
| Internal bar code: | GGCCAGACACGCTCCAGTGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 299 |
| LEAP-Seq percent confirming: | 99.6458 |
| LEAP-Seq n confirming: | 4220 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATTGGAGGAGCAAGAGTGC |
| Suggested primer 2: | GATACTCACATGCAATGGCG |