Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.156763 |
Chromosome: | chromosome_13 |
Location: | 3730489 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre13.g589350 | FBB6 | Flagellar/basal body protein | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTGCCTGCCCCTTTCTCTCACACCTTGGGA |
Internal bar code: | CGATCAGCTGCTCCGTTCGCCA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 815 |
LEAP-Seq percent confirming: | 97.5309 |
LEAP-Seq n confirming: | 158 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCTAGCTAGATAGCGCCCCT |
Suggested primer 2: | GGATGTCGCAGTGGATTCTT |