| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.156764 |
| Chromosome: | chromosome 9 |
| Location: | 7165405 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g412100 | PSAF1,PSAF | (1 of 1) K02694 - photosystem I subunit III (psaF); Photosystem I reaction center subunit III | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCGCAACCCGGCCGTCAAGGCTTCCTCG |
| Internal bar code: | AATGAATACTTCCGGGCCCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 32 |
| LEAP-Seq percent confirming: | 99.3976 |
| LEAP-Seq n confirming: | 165 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAACGTAGAGGAAGCCAA |
| Suggested primer 2: | GCAGGTCCCTCTTGTAACCA |