Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.156768 |
Chromosome: | chromosome 6 |
Location: | 2099967 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g265150 | (1 of 1) K11833 - ubiquitin carboxyl-terminal hydrolase 2/21 [EC:3.4.19.12] (USP2_21) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCATAGGATGAGGTGTAGCCTAGCGTGG |
Internal bar code: | TTCCTCGGCGAATTCGTTTCCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 206 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 69 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCTGCATTTCTCTAGCCTCG |
Suggested primer 2: | CCTATGCGGCTTCTGAAGAG |