| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.156768 |
| Chromosome: | chromosome 6 |
| Location: | 2099989 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g265150 | (1 of 1) K11833 - ubiquitin carboxyl-terminal hydrolase 2/21 [EC:3.4.19.12] (USP2_21) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCGTCACGCACAGGGGAAGAACCAAGTG |
| Internal bar code: | CTACAATTGGGGGCCCCCGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 353 |
| LEAP-Seq percent confirming: | 97.3503 |
| LEAP-Seq n confirming: | 1837 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCATTTCTCTAGCCTCG |
| Suggested primer 2: | CCTATGCGGCTTCTGAAGAG |