| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.156825 |
| Chromosome: | chromosome 4 |
| Location: | 4048418 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g232602 | GRX4 | Glutaredoxin 4, CGFS type; (1 of 1) PTHR10293:SF40 - GLUTAREDOXIN-3 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTACATAGATTCATGAGTGGCTGATCAT |
| Internal bar code: | TCGTGGTAGGGTTTGTCACATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 818 |
| LEAP-Seq percent confirming: | 99.9132 |
| LEAP-Seq n confirming: | 1151 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCCGCCCCTACCTACTCTC |
| Suggested primer 2: | ATGGTAGACAGCCGTGAAGC |