Insertion junction: LMJ.RY0402.156836_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.156836
Chromosome:chromosome_12
Location:7491496
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre12.g555950 DHC11 Putative flagellar inner arm dynein heavy chain, novel minor typ antisense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):ATGATCAATGAACATGTGAGAAATGGCGTG
Internal bar code:TTGTCGATGAAGTATAAACGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:526
LEAP-Seq percent confirming:99.7611
LEAP-Seq n confirming:2506
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TCACTGGTCTTGTACAGCGG
Suggested primer 2:GGATTTGGGAGTGGATGTTG