Insertion junction: LMJ.RY0402.156850_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.156850
Chromosome:chromosome 10
Location:3993712
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre10.g448900 (1 of 80) IPR003882 - Pistil-specific extensin-like protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCATTAACACCGCCGGGCTTTCGCCAACA
Internal bar code:AGCCTCCCATCCGCTGGCTGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:183
LEAP-Seq percent confirming:46.7047
LEAP-Seq n confirming:1474
LEAP-Seq n nonconfirming:1682
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:CAGCTCCTTCGTCATCAACA
Suggested primer 2:CACCGGCTTCTACTCAGACC