| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.156854 |
| Chromosome: | chromosome 17 |
| Location: | 5606977 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g738000 | AGG2 | Aggregation 2; (1 of 6) PTHR15907:SF21 - DUF614 FAMILY PROTEIN | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCATATCCTTCACCTGCCAGCTGCTAGGG |
| Internal bar code: | GGCGCGTAGCGACGGGGCGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1071 |
| LEAP-Seq percent confirming: | 99.8338 |
| LEAP-Seq n confirming: | 2403 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTTCCTGCTGTTTGGTTGG |
| Suggested primer 2: | TCGCTATGATCAGAACGTCG |