| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.156941 |
| Chromosome: | chromosome 1 |
| Location: | 6075103 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g043250 | (1 of 3) PTHR30570:SF0 - PHOSPHATE-BINDING PROTEIN PSTS | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTAGAGGACGCTGCATCTTTTGTACATG |
| Internal bar code: | GTTGGTTCTCTAGCAGTGGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1067 |
| LEAP-Seq percent confirming: | 99.7639 |
| LEAP-Seq n confirming: | 2535 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCAAACACAGGGTGTGGTG |
| Suggested primer 2: | AGTTCCGACCCGTTACACAG |