| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.156953 |
| Chromosome: | chromosome 1 |
| Location: | 1395642 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g006950 | FBA1 | (1 of 1) PTHR11627//PTHR11627:SF6 - FRUCTOSE-BISPHOSPHATE ALDOLASE // SUBFAMILY NOT NAMED; Fructose-1%252C6-bisphosphate aldolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGAATTTTGGGAGAGAGCGTTGAGTTAG |
| Internal bar code: | TGTTGGAATCACCCCTCGGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 823 |
| LEAP-Seq percent confirming: | 99.5878 |
| LEAP-Seq n confirming: | 1933 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCCTCTACTCCCTGAATC |
| Suggested primer 2: | AATGCCCGTTATTACCCTCC |