| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.156976 |
| Chromosome: | chromosome 12 |
| Location: | 9552257 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g546452 | GT90F45,GT90-45 | (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90); GT90 family protein 45 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGATGTAGGGACGGAATTCCAACGACTG |
| Internal bar code: | TTCGGCCTGCTGCCACCTCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 619 |
| LEAP-Seq percent confirming: | 98.0762 |
| LEAP-Seq n confirming: | 2549 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACAGGACCGGATTAGAAA |
| Suggested primer 2: | CTCACGAACCATCTCCACCT |