| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.156979 |
| Chromosome: | chromosome 16 |
| Location: | 804852 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g647534 | NRX4 | Nucleoredoxin 4; (1 of 3) K17609 - nucleoredoxin [EC:1.8.1.8] (NXN) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGATGCGGTACTGATGCAGTAGCTAAAGA |
| Internal bar code: | ACAAGTTTTGCGGCATTAGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 720 |
| LEAP-Seq percent confirming: | 99.6193 |
| LEAP-Seq n confirming: | 785 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTGTCAGGGGGATGAAAA |
| Suggested primer 2: | AGATGCCCTGGATGTCCATA |