| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.156990 |
| Chromosome: | chromosome 3 |
| Location: | 5112022 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g181650 | (1 of 1) PTHR20973//PTHR20973:SF0 - NON-SMC ELEMENT 1-RELATED // NON-STRUCTURAL MAINTENANCE OF CHROMOSOMES ELEMENT 1 HOMOLOG | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTCGCAGCTGAAACAGTTGGGAGGAAATG |
| Internal bar code: | ACGCGGTCGGTTTACACCATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 781 |
| LEAP-Seq percent confirming: | 99.5345 |
| LEAP-Seq n confirming: | 2138 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCAATGCTAGGAAATGGGC |
| Suggested primer 2: | GTGTGCAGCCATGCTCTTTA |