| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.156992 |
| Chromosome: | chromosome 2 |
| Location: | 1207876 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g082050 | (1 of 1) K13115 - coiled-coil domain-containing protein 130 (CCDC130) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTGGGCGTACCACCGGGAGATCTGGAGC |
| Internal bar code: | GCTCCCGCCGAGGGCAAAATCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 527 |
| LEAP-Seq percent confirming: | 99.8056 |
| LEAP-Seq n confirming: | 1540 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCAGGCAGACAGACAGAT |
| Suggested primer 2: | TGATGGGGTTCTTGTAAGCC |