| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.156996 |
| Chromosome: | chromosome 12 |
| Location: | 5906530 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g534100 | COL,CON2 | (1 of 3) PTHR31319//PTHR31319:SF10 - FAMILY NOT NAMED // ZINC FINGER PROTEIN CONSTANS-RELATED; CONSTANS-like protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCTGTAACACAACGCGCATGCGAGTACC |
| Internal bar code: | CTAGCAGAGGGGAAGAGGGCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 769 |
| LEAP-Seq percent confirming: | 99.902 |
| LEAP-Seq n confirming: | 3059 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTCAGGACGTGAGTTTGT |
| Suggested primer 2: | GCGACTGCGCACTTATAACA |