| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.156998 |
| Chromosome: | chromosome 1 |
| Location: | 4427242 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g030050 | PRPL34 | Chloroplast ribosomal protein L34; (1 of 1) K02914 - large subunit ribosomal protein L34 (RP-L34, MRPL34, rpmH) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGGCAGCAGCATGACGCGCGTGGCTCCC |
| Internal bar code: | CACTGGCGTGTCCGGACGCCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1002 |
| LEAP-Seq percent confirming: | 99.1471 |
| LEAP-Seq n confirming: | 465 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGGGCATTCATAGAAGCG |
| Suggested primer 2: | TAATCGAGGACGGCAAAATC |