| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.157019 |
| Chromosome: | chromosome_12 |
| Location: | 2057477 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g509800 | FAP286 | Flagellar Associated Protein | antisense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTTTGAAGCCAGGGGCCCCGGGAGGGGTCA |
| Internal bar code: | ACGGACCAACTGGCCAGACGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1150 |
| LEAP-Seq percent confirming: | 97.0669 |
| LEAP-Seq n confirming: | 7082 |
| LEAP-Seq n nonconfirming: | 214 |
| LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGTCCATAGATGTCCCGT |
| Suggested primer 2: | CGCATGTGCTGTTGCTAAAT |