| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.157019 |
| Chromosome: | chromosome 12 |
| Location: | 2057478 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g509800 | FAP286 | Flagellar Associated Protein 286 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAGCCATGAAGAAGGCGACGGAGGGGAA |
| Internal bar code: | TGGTGTGGACGGGCACAACCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 910 |
| LEAP-Seq percent confirming: | 98.5444 |
| LEAP-Seq n confirming: | 3385 |
| LEAP-Seq n nonconfirming: | 50 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCATGTGCTGTTGCTAAAT |
| Suggested primer 2: | GCTGTCCATAGATGTCCCGT |