| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.157023 |
| Chromosome: | chromosome 3 |
| Location: | 6008539 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g190850 | ECH1 | EnoylCoA hydratase/isomerase; (1 of 1) K13238 - 3,2-trans-enoyl-CoA isomerase, mitochondrial (DCI) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGTATACTTCGAACTTCCGGTGTGCAGG |
| Internal bar code: | CACGTGCTCGCTAAGCGTTCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 898 |
| LEAP-Seq percent confirming: | 99.2851 |
| LEAP-Seq n confirming: | 1111 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCCTAGCCACCAGCTTGA |
| Suggested primer 2: | TAGGGTTGGTGGATGACGTT |