| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.157069 |
| Chromosome: | chromosome 7 |
| Location: | 1559017 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g324400 | VPS24 | Subunit of the ESCRT-III complex; (1 of 1) K12193 - charged multivesicular body protein 3 (VPS24, CHMP3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCCCGATCACCTGCCGTCTCTGTGCTGG |
| Internal bar code: | ATGTGGCAGCATGGAGGGGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 455 |
| LEAP-Seq percent confirming: | 8.74471 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 647 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGGAAAGACCGATTGGAA |
| Suggested primer 2: | CCCAGACTTACCCTTGGACA |