| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.157086 |
| Chromosome: | chromosome 5 |
| Location: | 777914 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g248401 | (1 of 4) 3.4.21.19 - Glutamyl endopeptidase / V8 proteinase | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGACAATGCGAAGTCGAGGAAGCACTCGA |
| Internal bar code: | GGTTTAACCTCATTCTGCCTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 502 |
| LEAP-Seq percent confirming: | 99.6542 |
| LEAP-Seq n confirming: | 8933 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATACAACGCACAGGATTGG |
| Suggested primer 2: | TTCGAGGTGTGTGGTTGGTA |