| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.157099 |
| Chromosome: | chromosome 16 |
| Location: | 4745669 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g686750 | PTA3 | (1 of 10) IPR005828//IPR020846 - Major facilitator, sugar transporter-like // Major facilitator superfamily domain; Proton/phosphate symporter | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCCTCGCTGCTCCAATCCATAATCACAT |
| Internal bar code: | GTACTGAGACTCGTCCCGGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 848 |
| LEAP-Seq percent confirming: | 99.8008 |
| LEAP-Seq n confirming: | 1503 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCATCCTGCACAGTCCTGA |
| Suggested primer 2: | CTTTACGCTTCCGAATGAGC |