Insertion junction: LMJ.RY0402.157104_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.157104
Chromosome:chromosome 6
Location:8676428
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g309650 (1 of 1) IPR003200//IPR006626//IPR011050 - Nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase-like // Parallel beta-helix repeat // Pectin lyase fold/virulence factor CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAAATTGACAACCCCCAGCCCCCGCCGCGG
Internal bar code:AATCGAGGCGCTGGGATGATGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1015
LEAP-Seq percent confirming:95.8531
LEAP-Seq n confirming:15394
LEAP-Seq n nonconfirming:666
LEAP-Seq n unique pos:56

Suggested primers for confirmation by PCR

Suggested primer 1:CTCAGCAACAGAAGCAGCAG
Suggested primer 2:CATGCATTGGGTGCTCATAG