Insertion junction: LMJ.RY0402.157120_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.157120
Chromosome:chromosome 10
Location:3739659
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g446500 CPO1,CPL24 (1 of 1) PTHR14241//PTHR14241:SF10 - INTERFERON-INDUCED PROTEIN 44 // VON WILLEBRAND FACTOR A DOMAIN-CONTAINING PROTEIN DDB_G0292188; Conserved in the Plant Lineage intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTACTCAACACTCAACAGTGGAGCCTTCCA
Internal bar code:CGTGTAGATACCCTGCGCCTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:54
LEAP-Seq percent confirming:85.7143
LEAP-Seq n confirming:42
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CGTCCTATCGCACCTAGCTC
Suggested primer 2:ACACGTTCACCTGCCTATCC