| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.157150 |
| Chromosome: | chromosome 16 |
| Location: | 4697734 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g687117 | (1 of 2) PF14249 - Tocopherol cyclase (Tocopherol_cycl) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTGGTGGCGGCGGCCGAGCCACACTCCT |
| Internal bar code: | CAACGGGACCGACTATCGTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 884 |
| LEAP-Seq percent confirming: | 86.9565 |
| LEAP-Seq n confirming: | 160 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACAATCACCACACTGCCA |
| Suggested primer 2: | ACCAGTACTGCTGCACAACG |