| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.157189 |
| Chromosome: | chromosome 13 |
| Location: | 3224656 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g585700 | (1 of 42) IPR000008 - C2 domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTGCACCGCCCCGAACCCGGGCGGGTGC |
| Internal bar code: | TTAGGCGGTCCGGATGGCCCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 457 |
| LEAP-Seq percent confirming: | 35.9178 |
| LEAP-Seq n confirming: | 1853 |
| LEAP-Seq n nonconfirming: | 3306 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAAGAGTTGCTTTTCCCA |
| Suggested primer 2: | AACTCCTGCACACCCATTTC |