| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.157192 |
| Chromosome: | chromosome 10 |
| Location: | 5867101 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g461850 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCGCTTGGAAACAGGAAAGCTTTTTCGC |
| Internal bar code: | AAGCGGCAGGTGATTTGCTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 930 |
| LEAP-Seq percent confirming: | 98.4339 |
| LEAP-Seq n confirming: | 1697 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGCCGTAGAGCCAAAGTC |
| Suggested primer 2: | ATTTCCGTGTTGCGGTACTC |