Insertion junction: LMJ.RY0402.157207_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.157207
Chromosome:chromosome 6
Location:1018271
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g256050 (1 of 19) 3.6.1.3 - Adenosinetriphosphatase / Triphosphatase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCAGCTGTTTGCAGGGTTGCGCCCGACAC
Internal bar code:TTATTAAGATGTTGAGGTTCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:678
LEAP-Seq percent confirming:97.7427
LEAP-Seq n confirming:1732
LEAP-Seq n nonconfirming:40
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:GTAGCTGCTGTTTTGAGGGC
Suggested primer 2:GAGTCGCTGAGTCGCTTACC