Insertion junction: LMJ.RY0402.157226_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.157226
Chromosome:chromosome 14
Location:2746909
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre14.g627050 (1 of 17) IPR001305 - Heat shock protein DnaJ, cysteine-rich domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCTCACCTACAAACCCCCGCACCCCCACG
Internal bar code:CCGCGCCAGACGAGAGCCAAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:442
LEAP-Seq percent confirming:98.7971
LEAP-Seq n confirming:2546
LEAP-Seq n nonconfirming:31
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CAATTGACTTTGCCTGCTGA
Suggested primer 2:ACACACACTCCCTCTCACCC