Insertion junction: LMJ.RY0402.157237_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.157237
Chromosome:chromosome 11
Location:1519966
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g467747 (1 of 2) PF10173 - Mitochondrial K+-H+ exchange-related (Mit_KHE1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTGCGAGGACCCTATCGGCGCAGAGACAC
Internal bar code:ACCACACATCAGCGGTCTTCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:754
LEAP-Seq percent confirming:99.8889
LEAP-Seq n confirming:2698
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:CCGGATGCATTACAAAATCC
Suggested primer 2:TTCGTTCTTCGAGCAATGTG