| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.157241 |
| Chromosome: | chromosome 12 |
| Location: | 9014832 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g543550 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAATGGCTGCGTGTCGTCCCAAGGCGGC |
| Internal bar code: | TCGATGGAGCTTCGAATGCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 738 |
| LEAP-Seq percent confirming: | 77.1429 |
| LEAP-Seq n confirming: | 135 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTCAACCATGTGAAACCG |
| Suggested primer 2: | CTGTCGGGCCATAAGAATGT |