| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.157312 |
| Chromosome: | chromosome 9 |
| Location: | 6624749 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g408600 | (1 of 1) 1.13.11.29 - Stizolobate synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTGAGGTTGAGGTAATGCGGTTGAGGTA |
| Internal bar code: | GCAGGCCACACTCGGCCAGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 831 |
| LEAP-Seq percent confirming: | 81.4464 |
| LEAP-Seq n confirming: | 2151 |
| LEAP-Seq n nonconfirming: | 490 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCCATGTACCCTCCATTC |
| Suggested primer 2: | TTCACGCTTAAGAAACGGCT |