| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.157314 |
| Chromosome: | chromosome 12 |
| Location: | 5736326 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g532950 | (1 of 4) PF00060//PF00497 - Ligand-gated ion channel (Lig_chan) // Bacterial extracellular solute-binding proteins, family 3 (SBP_bac_3); Ligand-gated ion channel | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGATCATAGGGTTGAGGGAAGCGGGGCA |
| Internal bar code: | CGGGTCTAGGGATACGTCGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1105 |
| LEAP-Seq percent confirming: | 87.5472 |
| LEAP-Seq n confirming: | 2320 |
| LEAP-Seq n nonconfirming: | 330 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAACGCCGAATAGTAAGC |
| Suggested primer 2: | CATCCCTTCCCTCCTCTTTC |