| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.157329 |
| Chromosome: | chromosome 10 |
| Location: | 2149225 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g433550 | DNL4,LIG4,LIG2 | (1 of 21) IPR001357 - BRCT domain; DNA ligase 4 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGTCGAAAACCCAAGGTCCTTTGGCCCTG |
| Internal bar code: | TGGGCTAAACCGGAACTTGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 41 |
| LEAP-Seq percent confirming: | 43.5484 |
| LEAP-Seq n confirming: | 54 |
| LEAP-Seq n nonconfirming: | 70 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACACAAGCACAACCAACCA |
| Suggested primer 2: | CCACATACCGCCTCAAACTT |