Insertion junction: LMJ.RY0402.157334_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.157334
Chromosome:chromosome 1
Location:2934132
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre01.g017900 (1 of 8) IPR011047//IPR018391 - Quinonprotein alcohol dehydrogenase-like superfamily // Pyrrolo-quinoline quinone beta-propeller repeat intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCGCAACAGCGCTACCGTGCGACTATGCT
Internal bar code:GATTCGAGATTTATCGCTACAC

Confirmation - LEAP-Seq

LEAP-Seq distance:49
LEAP-Seq percent confirming:99.8192
LEAP-Seq n confirming:552
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GGGTGCAGCAATACTTTGGT
Suggested primer 2:TTCCGCTGACTTCTTCGTTT