Insertion junction: LMJ.RY0402.157342_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.157342
Chromosome:chromosome 6
Location:3684160
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g278140 (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGGCTTTGTGGTGTAAGATGGGAAGATG
Internal bar code:CGTTACATGTTTGCGGCTGAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:863
LEAP-Seq percent confirming:97.2152
LEAP-Seq n confirming:1152
LEAP-Seq n nonconfirming:33
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CAAGAACGAAAAGGACGCTC
Suggested primer 2:AGCCATACAAAGCTGCCTGT