Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.157350 |
Chromosome: | chromosome 16 |
Location: | 1454304 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre16.g652600 | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTCCACCAGCCGCGCCCGGTCACTCTGG |
Internal bar code: | GTGCGGACATTCGTCCGGGGGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 457 |
LEAP-Seq percent confirming: | 99.6288 |
LEAP-Seq n confirming: | 1342 |
LEAP-Seq n nonconfirming: | 5 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAACACTGTCCCACCATCAC |
Suggested primer 2: | CAGCTCTATTGCGCTGTCAC |