Insertion junction: LMJ.RY0402.157350_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.157350
Chromosome:chromosome 16
Location:1454304
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g652600 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGTCCACCAGCCGCGCCCGGTCACTCTGG
Internal bar code:GTGCGGACATTCGTCCGGGGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:457
LEAP-Seq percent confirming:99.6288
LEAP-Seq n confirming:1342
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CAACACTGTCCCACCATCAC
Suggested primer 2:CAGCTCTATTGCGCTGTCAC