| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.157351 |
| Chromosome: | chromosome 9 |
| Location: | 5219227 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g399402 | (1 of 2) PTHR24305//PTHR24305:SF65 - FAMILY NOT NAMED // CYTOCHROME P450 12A4, MITOCHONDRIAL-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCTGACCTCCATACCCACACGGCTGCTG |
| Internal bar code: | GAGTATCAACACCGATGGGAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 835 |
| LEAP-Seq percent confirming: | 96.4524 |
| LEAP-Seq n confirming: | 3752 |
| LEAP-Seq n nonconfirming: | 138 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCTCTCATAGTCGGAGGC |
| Suggested primer 2: | CCCTCTGTAACATCCGCATT |