Insertion junction: LMJ.RY0402.157384_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.157384
Chromosome:chromosome 14
Location:2930271
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g627576 DHC7 (1 of 2) PTHR10676//PTHR10676:SF136 - DYNEIN HEAVY CHAIN FAMILY PROTEIN // DYNEIN HEAVY CHAIN 6, AXONEMAL; Axonemal Dynein Heavy Chain 7 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTTTATGCTTGGGGCTGGGTGGCGTGGGG
Internal bar code:TAAAGCGGCGCCGTGAGGTAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:217
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:274
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CGTGGAGAAGCTTCAGTTCC
Suggested primer 2:CTCTCCCCCAACGTCATAAA