| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.157401 |
| Chromosome: | chromosome_2 |
| Location: | 6100275 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g113200 | GLN1,NSG18 | Glutamine synthetase | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGGTGCGCCAGCTGGTCACACATCAACCCT |
| Internal bar code: | GTCTAATTAGACTAGCTGGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 786 |
| LEAP-Seq percent confirming: | 98.5577 |
| LEAP-Seq n confirming: | 205 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAGTCGGGGATTTCTTCT |
| Suggested primer 2: | TGCTCACGCATGTAGACACA |