| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.157407 |
| Chromosome: | chromosome_2 |
| Location: | 4724745 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g102050 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AATCTTGGACACCTGGGTGGGCTCCTTGAA |
| Internal bar code: | GCTACAAGTGCGGATGGCGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 439 |
| LEAP-Seq percent confirming: | 99.5614 |
| LEAP-Seq n confirming: | 1135 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTGACGGAAAGGGGTACA |
| Suggested primer 2: | CTCTGTGTTGCCGATGCTTA |