| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.157421 |
| Chromosome: | chromosome 16 |
| Location: | 2413929 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g660350 | (1 of 1) K15363 - fanconi-associated nuclease 1 (FAN1, MTMR15) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGCAGCTGCTGCTGGAGCGGGTGAGGCG |
| Internal bar code: | TATCTAAGTTTAGTATATATCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1000 |
| LEAP-Seq percent confirming: | 99.2408 |
| LEAP-Seq n confirming: | 1830 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTACAGTTGTGCTGCCAAA |
| Suggested primer 2: | TTTACTGCAGTCCCAAACCC |