| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.157430 |
| Chromosome: | chromosome 10 |
| Location: | 187079 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g419000 | (1 of 1) PTHR13105//PTHR13105:SF7 - MYELOID LEUKEMIA FACTOR // MYELOID LEUKEMIA FACTOR | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGATTTTATTGTCGGGAAGCTGCAGTGG |
| Internal bar code: | CTGCTGGGGCTCGTCCACACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 690 |
| LEAP-Seq percent confirming: | 99.3798 |
| LEAP-Seq n confirming: | 37014 |
| LEAP-Seq n nonconfirming: | 231 |
| LEAP-Seq n unique pos: | 152 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTTACGGGAGGGTACATGC |
| Suggested primer 2: | ACACACACACCACACACACG |