| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.157435 |
| Chromosome: | chromosome_17 |
| Location: | 1212289 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g705000 | CDPK1 | Calcium-dependent protein kinase | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AAGAGCCTAACGCAGCGCGGCTGCACCGTA |
| Internal bar code: | CTGGGATAATTAAAGAGGGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 902 |
| LEAP-Seq percent confirming: | 93.819 |
| LEAP-Seq n confirming: | 850 |
| LEAP-Seq n nonconfirming: | 56 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCACAGTCCGCATCTGTTC |
| Suggested primer 2: | GACAATCAGCAGCTTGACCA |