| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.157438 |
| Chromosome: | chromosome 9 |
| Location: | 2563488 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g390700 | POB1 | Proteome of basal body 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGTAGTACACTGGCGGCGGCTCCGGGCG |
| Internal bar code: | GGGCTCAACGGACGGAGGGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 92 |
| LEAP-Seq percent confirming: | 58.1967 |
| LEAP-Seq n confirming: | 71 |
| LEAP-Seq n nonconfirming: | 51 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCACACACACACACACAC |
| Suggested primer 2: | AAGAGGAGGACGAGGGAGAG |