Insertion junction: LMJ.RY0402.157438_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.157438
Chromosome:chromosome_9
Location:2563488
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre09.g390700 sense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):GCTAACGGTCAACCATACGTGCTGAGCCGC
Internal bar code:CCGGCAACGGTCGCGATACGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:161
LEAP-Seq percent confirming:17.3176
LEAP-Seq n confirming:470
LEAP-Seq n nonconfirming:2244
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:AAGAGGAGGACGAGGGAGAG
Suggested primer 2:ACGCACACACACACACACAC