| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.157441 |
| Chromosome: | chromosome_12 |
| Location: | 5840306 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g533650 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGAATGGAGAACTTGAACCCCAAGAGTGGC |
| Internal bar code: | CTTCTGATCACATTGATCTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1319 |
| LEAP-Seq percent confirming: | 98.6639 |
| LEAP-Seq n confirming: | 960 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTATGAGCGAATGGTGGA |
| Suggested primer 2: | GTGTTGCCTTGCGAAGTACA |