| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.157442 |
| Chromosome: | chromosome 2 |
| Location: | 155219 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g074300 | (1 of 1) PF11707 - Ribosome 60S biogenesis N-terminal (Npa1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGGTGAACCGGGTTGCAGATTGTTGAGG |
| Internal bar code: | AGGCACTGATGCCGCCTATGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1084 |
| LEAP-Seq percent confirming: | 98.2307 |
| LEAP-Seq n confirming: | 4497 |
| LEAP-Seq n nonconfirming: | 81 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCCTTCTCCACCTCATGTC |
| Suggested primer 2: | GGGTACATATGACTGCGGCT |